Continuous sedimentation coefficient distributions of the monomeric PAD4 interface mutants.

<p>The enzyme concentrations used in the experiments were 0.1, 0.3 and 1 mg/mL in 50 mM Tris-HCl and 250 mM NaCl (pH 7.6) at 20°C. (<b>A</b>) WT; (<b>B</b>) R8L mutant; (<b>C</b>) R8E mutant; (<b>D</b>) R8E/D547E double mutant; (<b>E</b>) Y435A mutant; (<b>F</b>) Y435N mutant.