Effect of the composition of the external medium on the inhibition of double cysteine mutants by CuPh.

<p>HeLa cells expressing double cysteine mutants were preincubated for 5 min in the presence and absence of 200 µM CuPh. The indicated preincubation solutions contained NaCl, NaCl +1 mM L-glutamate, ChCl +1 mM L-glutamate, NaCl +1 mM GABA, NaCl +1 mM glycine, NaCl +20 µM TBOA, KCl, choline chloride. Values are given as percent of control (preincubation without CuPh) and represent the mean ± S.E. of at least three different experiments done in triplicate. (<b><i>A</i></b>) I295C/I463C double cysteine mutants. (<b><i>B</i></b>) G297C/I463C double cysteine mutants.