Influence of salt concentration on the inhibition of protein synthesis by PI-103.

<p>4E-BP wild-type and DKO cells were incubated in complete medium in the absence or presence of additional 0.1 M NaCl. After 1 h the cells were further incubated with or without PI-103 (5 µM) for 1 h and total protein synthesis was then measured by the incorporation of [³⁵S]methionine (2 µCi/ml) for 1 h as described in Experimental. The data are the means ± S.E.M of 9 independent determinations on 4E-BP wild-type cells and 6 independent determinations on DKO cells and are expressed as the % inhibition of protein synthesis by PI-103 in the absence or presence of the additional NaCl. Significance of difference was determined by unpaired t test: * = p<0.01.