Inhibition of transport of cysteine mutants by Cd^2<b>+</b>.

<p>HeLa cells expressing the indicated mutants were washed once with choline chloride-containing solution and assayed for transport in the presence or absence of 500 µM cadmium chloride. Values shown are the percentage activity in the presence of 500 µM cadmium chloride relative to that in its absence. Values represent the mean ± S.E. of at least three separate experiments each done in triplicate. (<b><i>A</i></b>) I295C/I463C double cysteine mutants and its control mutants. (<b><i>B</i></b>) G297C/I463C double cysteine mutants and its control mutants.