Oxidative stress in cells infected with MP12 strain of RVFV.

<p>A) HSAECs were infected with MP12 virus (MOI of 3) and 24 h later stained with 3 µg/ml of Mitosox reagent. After incubation for 30 min the Mitosox reagent was removed, cells washed 3× with HBSS, fixed with paraformaldehyde, visualized. Boxed and magnified inset shows Mitosox staining inside nucleoli. B) MP12 infected HSAECs were stained with Mitosox reagent at 4, 6, 12 and 24 h post infection and visualized as described earlier. C) Mean fluorescence intensity was calculated by averaging flouresence in ten fields picked randomly from quadruplicate samples for every time point. The mean fluorescence intensity of the infected samples was then calculated as fold difference over uninfected cells obtained at comparable time points. * p<0.00005. D) HSAECs were pre- and post treated with DMSO or increasing concentrations of NAC. Untreated and treated cells were infected with MP12 virus and SOD1 protein levels were analyzed by western blot with SOD1 antibody. Actin was used as a control.