Effects of RV infection on cell proliferation and mitosis.

<p>(<b>A</b>) Growth curves of mock-infected and RV-infected HUVECs. HUVEC were mock infected or infected with RV-Dz at MOI=10 and then counted daily. The data are results of 2 independent experiments each performed in duplicate. (<b>B</b>) Mitosis in infected HUVEC. At 2 dpi the mock infected and infected HUVEC (RV-Dz, MOI=10) were immunostained by IFA with capsid MAb and DAPI to quantitate infected cells and mitotic figures. Mitotic indexes (MI) were calculated as % cells with mitotic figures in two duplicate wells. Note RV-antigen positive mitotic cell in red circle. (<b>C</b>) Cell cycle analysis of infected HUVEC. Serum-starved HUVECs were mock-infected or infected with RV-Dz at MOI=10. Histograms of cell cycle analysis at 1 dpi show DNA content of propidium iodide-stained cells by flow cytometry and % of cells in each phase of the cell cycle. The representative results of two independent experiments are shown.