Relationship between MuV-induced SG formation, IFN production and viral replication in PKR-KD and RLR-related protein-KD cells.

<p>FL cells were transfected with targeting siRNA (PKR si, MDA5 si, RIG-I si, or MAVS si) or nontargeting control siRNA (N.C. si) for 48 h, and then infected with MuV at MOI 0.5. (A) Immunofluorescence staining of G3BP1 (red) and MuV P/V proteins (green) at 24 h post-infection of MuV. Nuclei were stained with DAPI (blue). Scale bar indicates 20 μm. (B) Western blotting of whole cell lysates derived from cells at 24 h post-infection. β-Actin was determined as a control. (C) IFNs (<i>n</i> = 4) determined by ELISA and (D) viral titer (<i>n</i> = 4) determined by plaque-forming assay in culture supernatants at 24 h post-infection at MOI 0.5. The bar graphs represent means ± standard deviation. *<i>P</i><0.05, **<i>P</i><0.01 versus MuV-infected control cells (N.C. siRNA-transfected cells).