Host antiviral responses restrict MuV propagation in RPAP3-knockdown cells.

At 48 h post-transfection with either siRPAP3 or siNC, A549/SeV-C and A549/Ctrl cells were infected with rMuV/AcGFP at a MOI of 0.05. (A) Supernatants were collected at 0, 24, 48, 72 and 96 hpi, and infectious titers were determined by plaque assay. (B) At 72 hpi, cells were observed under a fluorescence microscope. (C) At 72 hpi, cell lysates were immunoblotted with the indicated antibodies. The relative band intensities of each viral protein were normalized to β-actin level, and the relative expression of each protein based on levels in siNC-treated A549/Ctrl cells is shown. Error bars indicate the standard deviations of triplicate wells.