DNMT and HDAC inhibition impairs or enhances, respectively, amygdala LTP at thalamic and cortical inputs.

<p>(A–B) Schematic of amygdala slice preparation for “thalamic” and “cortical” stimulation experiments, showing placement of stimulating and recording electrodes. (<i>C</i>) Mean (±SEM) percent field potential amplitude (relative to baseline) in slices treated with vehicle (n = 7; black circles) or 30 µM 5-AZA (n = 5; gray circles) followed by LTP induction at thalamic inputs. Traces from an individual experiment before and 30 min following tetanic stimulation and baseline transmission following 20 min of bath application of drug are shown in the <i>inset</i>. Scale, 0.2 mV by 10 msec. (<i>D</i>) Mean (±SEM) percent field potential amplitude (relative to baseline) in slices treated with vehicle (n = 6; black circles) or 30 µM 5-AZA (n = 7; gray circles) followed by LTP induction at cortical inputs. Traces from an individual experiment before and 30 min following tetanic stimulation and baseline transmission following 20 min of bath application of drug are shown in the <i>inset</i>. Scale, 0.2 mV by 10 msec. (<i>E</i>) Mean (±SEM) percent field potential amplitude (relative to baseline) in slices treated with vehicle (n = 10; black circles) or 2.5 µM TSA (n = 7; gray circles) followed by LTP at thalamic inputs. Traces from an individual experiment before and 30 min following tetanic stimulation and transmission following 20 min of bath application of drug are shown in the <i>inset</i>. Scale, 0.2 mV by 10 msec. (<i>F</i>) Mean (±SEM) percent field potential amplitude (relative to baseline) in slices treated with vehicle (n = 6; black circles) or 2.5 µM TSA (n = 7; gray circles) followed by LTP at cortical inputs. Traces from an individual experiment before and 30 min following tetanic stimulation and transmission following 20 min of bath application of drug are shown in the <i>inset</i>. Scale, 0.2 mV by 10 msec. (<i>G</i>) Mean (±SEM) percent field potential amplitude (relative to baseline) in slices treated with vehicle (n = 6; black circles) or 30 µM 5-AZA+2.5 µM TSA (n = 6; gray circles) followed by LTP at thalamic inputs. Traces from an individual experiment before and 30 min following tetanic stimulation and transmission following 20 min of bath application of drug are shown in the <i>inset</i>. Scale, 0.2 mV by 10 msec. (<i>H</i>) Mean (±SEM) percent field potential amplitude (relative to baseline) in slices treated with vehicle (n = 6; black circles) or 30 µM 5-AZA+2.5 µM TSA (n = 5; gray circles) followed by LTP at cortical inputs. Traces from an individual experiment before and 30 min following tetanic stimulation and transmission following 20 min of bath application of drug are shown in the <i>inset</i>. Scale, 0.2 mV by 10 msec.