NMDAR subunits modification in hippocampal slices after transcription or translation inhibition during LTP induction. <i>A.</i>

<p><i> Left:</i> Normalized slopes of evoked fEPSPs recorded as those in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0055244#pone-0055244-g003" target="_blank">Figure 3</a>, corresponding to the first pulse of the paired stimulation applied before and after TBS (arrow). Plots represent the average of five independent experiments over 90 minutes of recording (n = 5 for each group). <i>Black line</i>: drug perfusion. <i>Insert on top</i>: average traces of 10 individual recordings from a control slice and slices treated with ActD or CHX (black: 5 minutes before TBS; grey: 5 last minutes of recording). <i>Right:</i> Bars represent averages of normalized first pulse slopes of the 5 last minutes of recording for each group. LTP induction was blocked by 40 µg/ml CHX treatment (* p<0.05, one sample t test) compared to basal transmission (line referred to 1 in the graph). <b><i>B.</i></b> NMDAR subunits were evaluated by WB in same slices that in <b>A.</b> CHX treatment blocked GluN1 and GluN2A increase, while 50 µg/ml ActD only blocked GluN1 increase (* p<0,05 ONE WAY ANOVA, Dunnet Post-Test; n = 5 for each group). <i>Insert on top:</i> (from left to right): Representative GluN1 and GluN2A WB bands of +TBS+LTP slices (control), CHX and ActD +TBS+LTP slices treated slices. <b><i>C.</i></b> Table indicates mean ± SEM for GluN1/GAPDH (first row) or GluN2A/GAPDH (second row) in +TBS-LTP slices (n = 6) and +TBS+LTP slices without any drug treatment (Control in <b>B,</b> n = 9), or treated with ActD (n = 5) or CHX (n = 5) (*** p<0.0001; ONE WAY ANOVA - Newman Keuls Test).