Localization of PLP in HOG and GFP-MAL2-overexpressing HOG cells.

<p>HOG cells (A) or GFP-MAL2/HOG cells (B) cultured in GM or DM were fixed and processed for confocal double-label indirect immunofluorescence analysis with anti-PLP monoclonal antibody and P239 anti-MAL2 polyclonal antibody (A) or anti-PLP antibody alone (B). Monoclonal and policlonal antibodies were detected using Alexa Fluor 555 and Alexa Fluor 488 secondary antibodies respectively. Arrows point to accumulations of PLP in myelin-like sheets. GFP-MAL2-positive vesicles containing PLP can be seen close to the plasma membrane (square and arrowheads, the square show an enlarged region). Images correspond to the projection of the planes obtained by confocal microscopy. (DIC: differential interference contrast). Scale bars = 5 µm.