Co-immunoprecipitation of PLP and GFP-MAL2.

<p>Extracts from GFP-MAL2/HOG cells were immunoprecipitated with a monoclonal anti-PLP antibody (panel A) or with a polyclonal anti-GFP antibody (panel B) and incubated with protein A-Sepharose. Preimmune mouse or rabbit serum was used as a control (C). The whole cell lysate and the supernatants (SUP) recovered after centrifugation of the immunoprecipitates are also shown. Samples were analyzed by immunoblotting with monoclonal anti-PLP or polyclonal anti-GFP primary antibodies. Asterisks indicate height of relevant bands.