EM immunocolocalization of PLP and GFP-MAL2.

<p>Cryosections of GFP-MAL2/HOG cells cultured in DM at 37°C were stained with a rabbit polyclonal anti-GFP antibody and a mouse monoclonal anti PLP antibody. Anti-GFP and anti-PLP were detected with 10 nm protein A-gold and 15 nm anti-mouse-gold, respectively. Colocalization of PLP and GFP-MAL2 was observed in vesicles close to the plasma membrane (A); in scattered cytoplasmic vesicles (B); in tubulovesicles near the nucleus (C) and in tubulovesicular membrane-bound compartments (D). PM, plasma membrane; N, nucleus. Arrows point to 15 nm-gold particles. Scale bars = 100 nm.