<p>Lane 1–3: 10,000 parasites, Lane 4–6: 1000 parasites, Lane 7–9: 100 parasites, Lane 10–12: 10 parasites, Lane 13–15: 1 parasite, Lane 16–18: No Template controls (NTC), M = 100 bp marker.</p
<p>Parasitemia was assessed retrospectively by real-time quantitative PCR (qPCR) twice daily from da...
<p>Lane 1: ladder. Lanes 2 to 5: templates from 1 to 1000X diluted samples amplified for 40 cycles (...
<p>Solid dots represent PCR positive and hollow dots represent PCR negative samples. Detection limit...
<p>Lanes 2 to 4 show the PCR products for three samples which were negative for Madurella mycetomati...
<p>(A) PCR verification of expected 206 bp target length amplicon. Lane M, 50 bp DNA ladder (Molecul...
<p>The first lane (lane L) contains ladder (100 bp marker) for comparison of PCR products and for de...
<p>Comparison of parasite DNA quantity was made between (1) microscopy positive and negative samples...
<p>The second PCR products were visualized on 2% agarose-TAE gel containing GelRed (Wako). The prime...
<p>Parasite load in VL blood samples (Parasites/ml blood) and in BMA samples (Parasites/million nucl...
<p>PCR performance in confirmed cases (n = 20) compared to cases with other parasitosis (n = 7).</p
<p>Peak parasitemia (A, one-way ANOVA p = 0.13), geometric mean parasitemia during the first blood s...
<p>Lane 1∶100–1000 bp DNA ladder, Fermentas Lane 2: <i>Mecistocirrus digitatus</i> DNA (182 bp) Lane...
<p>NC: negative control; L: 100 bp Ladder; PC: positive control; lines 2, 3, 4, 5, 6, and 7: <i>S. m...
<p>Lanes H1and H2: Non-endemic healthy controls, Lane N1 and N2: negative controls, Lanes P1 to P8: ...
<p>Each row represents one sample. Pan, <i>Plasmodium spp</i>.; F, <i>P</i>. <i>falciparum</i>; M, <...
<p>Parasitemia was assessed retrospectively by real-time quantitative PCR (qPCR) twice daily from da...
<p>Lane 1: ladder. Lanes 2 to 5: templates from 1 to 1000X diluted samples amplified for 40 cycles (...
<p>Solid dots represent PCR positive and hollow dots represent PCR negative samples. Detection limit...
<p>Lanes 2 to 4 show the PCR products for three samples which were negative for Madurella mycetomati...
<p>(A) PCR verification of expected 206 bp target length amplicon. Lane M, 50 bp DNA ladder (Molecul...
<p>The first lane (lane L) contains ladder (100 bp marker) for comparison of PCR products and for de...
<p>Comparison of parasite DNA quantity was made between (1) microscopy positive and negative samples...
<p>The second PCR products were visualized on 2% agarose-TAE gel containing GelRed (Wako). The prime...
<p>Parasite load in VL blood samples (Parasites/ml blood) and in BMA samples (Parasites/million nucl...
<p>PCR performance in confirmed cases (n = 20) compared to cases with other parasitosis (n = 7).</p
<p>Peak parasitemia (A, one-way ANOVA p = 0.13), geometric mean parasitemia during the first blood s...
<p>Lane 1∶100–1000 bp DNA ladder, Fermentas Lane 2: <i>Mecistocirrus digitatus</i> DNA (182 bp) Lane...
<p>NC: negative control; L: 100 bp Ladder; PC: positive control; lines 2, 3, 4, 5, 6, and 7: <i>S. m...
<p>Lanes H1and H2: Non-endemic healthy controls, Lane N1 and N2: negative controls, Lanes P1 to P8: ...
<p>Each row represents one sample. Pan, <i>Plasmodium spp</i>.; F, <i>P</i>. <i>falciparum</i>; M, <...
<p>Parasitemia was assessed retrospectively by real-time quantitative PCR (qPCR) twice daily from da...
<p>Lane 1: ladder. Lanes 2 to 5: templates from 1 to 1000X diluted samples amplified for 40 cycles (...
<p>Solid dots represent PCR positive and hollow dots represent PCR negative samples. Detection limit...
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