PCR verification, detection limit and specificity using outer primers F3 and B3.

<p>(A) PCR verification of expected 206 bp target length amplicon. Lane M, 50 bp DNA ladder (Molecular weight marker XIII, Roche); lane Sm, <i>S. mansoni</i> DNA (1 ng); lane N, negative control (no DNA template). (B) Detection limit of PCR. Lane M, 50 bp DNA ladder (Molecular weight marker XIII, Roche); lane Sm: <i>S. mansoni</i> DNA (1 ng); lanes 10⁻¹–10⁻⁹: 10-fold serially dilutions of <i>S. mansoni</i> DNA; lane N, negative control (no DNA template). (C) Specificity of PCR. Lane M, 50 bp DNA ladder (Molecular weight marker XIII, Roche); lanes Sm, Sh, Sj, Si, Fh, Dd, Hd, Cd, Ll, Bp, As, Ts, Tt, Eg, Cp, Gd, Eh, <i>S. mansoni</i>, <i>S. haematobium</i>, <i>S. japonicum</i>, <i>S. intercalatum</i>, <i>Dicrocoelium dendriticum</i>, <i>Hymenolepis diminuta</i>, <i>Calicophoron daubneyi</i>, <i>Loa loa</i>, <i>Brugia pahangi</i>, <i>Anisakis simplex</i>, <i>Trichinella spiralis</i>, <i>Taenia taeniformis</i>, <i>Echinococcus granulosus</i>, <i>Cryptosporidium parvum</i>, <i>Giardia intestinalis</i> and <i>Entamoeba histolytica</i> DNA samples (1 ng/each), respectively; lane N, negative control (no DNA template).