Boxplots showing the amount of parasite DNA detected by SYBR quantitative polymerase chain reaction (QPCR) analysis of subset samples that were diagnosed as positive by conventional PCR.

<p>Comparison of parasite DNA quantity was made between (1) microscopy positive and negative samples; and (2) lowland and highland samples. Numbers above bars indicate number of individuals included. The central box represents the interquartile range and the whiskers represent the first quartile and the fourth quartile of the data. The median is shown as a lien through the center of the box and the ends of the whiskers correspond to the minimum and maximum in the data.