CD4⁺ effector memory T lymphocytes produce IFN-γ in response to rHBHAms.

<p>The phenotypic characteristics of the cells responding to the rHBHAms in the HBHA-responders were evaluated. As shown in a representative subject, a significant IFN-γ response to the rHBHAms was observed for CD4⁺ T-cells (<b>D</b>) over the negative control (<b>B</b>), whereas no response was detected for CD8⁺ T-cells (<b>C</b>) over the negative control (<b>A</b>). To characterize this immune response, naive and memory phenotypes were studied. Most of the CD4⁺ T-cells IFN-γ responding to the rHBHAms presented an effector memory phenotype (84%) defined as CD45R0⁺CD62L (<b>E</b>).