Memory phenotype of MTB-specific CD4 T cells using HLA class II tetramers.

<p>(A) HLA class II tetramer stained CD4-purified cells from LTBI donors. Tetramer⁺ cells were isolated following magnetic bead enrichment. Plots are gated on CD4⁺ T cells, and the numbers indicate the percentage of tetramer⁺ cells isolated from each of 4 representative donors CD4⁺ population. DPB1*04:01 AGCQTYKWETFLTSE n = 4 donors, DRB1*15:01 MHVSFVMAYPEMLAA n = 3, DRB1*15:01 MSQIMYNYPAMMAHA n = 5 and DRB1*01:01 GEEYLILSARDVLAV n = 2. (B) Memory phenotype of tetramer⁺ cells for one representative donor per tetramer. Plots are gated on total CD4⁺ T cells (black background) or epitope-specific CD4⁺ T cells (red dots). The numbers represent the percentages of tetramer⁺CD4⁺ T cells in the gate. (C) Scatter plot of the proportion of CCR7⁻CD45RA⁻ (effector memory), CCR7⁺CD45RA⁻ (central memory), CCR7⁺CD45RA⁺ (naïve), and CCR7⁻CD45RA⁺ (effector) CD4⁺ T cells for each tetramer. Each dot represents one donor/tetramer combination, median ± interquartile range is indicated.