<p>Mice of the indicated genotypes were treated <i>i.p.</i> with 1 mg IPTG and levels of Venus<sup>+</sup> cells in T-, B- and myeloid subsets in the peripheral blood were quantified by FACS analysis following the indicated times. Results of three independent experiments and 3 (VLV and LacI) or 6 (VLV/LacI) animals/genotype are represented as box plots. Box length equals interquartile range. Circles represent minimal and maximal values; *p<0.001; #p<0.05. F2 generation offspring of VLV founder A3 were crossed with LacI mice. Single-, double-, and non-transgenic littermates were used for analysis.</p
<p>(A) Schematic representation of transgene construction. (B) Southern blot analysis performed on 1...
<p>(A) PCR for detecting TG mice. Two pairs of primers were used to amplify the 489 bp and 282 bp in...
<p><b>A</b>) Representative images of groups of mice (n = 3 per group) transiently transgenized with...
<p>Primary cells derived from peripheral blood of VLV/LacI double transgenic mice were put in cultur...
<p>Animals that proved positive for Venus expression in the peripheral blood were sacrificed for fur...
<p>The <i>VavLacOVenus</i> (VLV) plasmid as well as an unmodified version of the <i>VavP</i> plasmid...
<p>(A) Genomic PCR for the βhCG transgene, using DNA derived from the peripheral blood cells of F1 m...
<p>(A) LN cells from B6 mice (WT), <i>Tcra</i><sup>−/−</sup> mice transgenic for Vα2 and Vα3.2 minil...
<p>Cells were harvested from the spleen and the lymph nodes of PbT-I transgenic or littermate contro...
<p>(A) Vα2, Vα3.2 and Vβ5 expression in lymph node T cells from B6 mice (WT), <i>Tcra</i><sup>−/−</s...
<p>A. Electron micrographs of RPEs from positive-strong transgenic mice lines 131, 142, and 157. B. ...
<p>(A) Transgenic Tg(Jpx) and Tg(Jpx, Xist) mouse lines generated in this study. F, female; M, male....
<p>(<b>A</b>) ES cells were transduced with different amounts of lentiviral NT or OT or were not tra...
<p>(A) Schematic map of the microinjected transgene consisting of the α-smooth muscle actin promoter...
<p>A. Schematic representation of the construct used for the generation of the transgenic mice. B. G...
<p>(A) Schematic representation of transgene construction. (B) Southern blot analysis performed on 1...
<p>(A) PCR for detecting TG mice. Two pairs of primers were used to amplify the 489 bp and 282 bp in...
<p><b>A</b>) Representative images of groups of mice (n = 3 per group) transiently transgenized with...
<p>Primary cells derived from peripheral blood of VLV/LacI double transgenic mice were put in cultur...
<p>Animals that proved positive for Venus expression in the peripheral blood were sacrificed for fur...
<p>The <i>VavLacOVenus</i> (VLV) plasmid as well as an unmodified version of the <i>VavP</i> plasmid...
<p>(A) Genomic PCR for the βhCG transgene, using DNA derived from the peripheral blood cells of F1 m...
<p>(A) LN cells from B6 mice (WT), <i>Tcra</i><sup>−/−</sup> mice transgenic for Vα2 and Vα3.2 minil...
<p>Cells were harvested from the spleen and the lymph nodes of PbT-I transgenic or littermate contro...
<p>(A) Vα2, Vα3.2 and Vβ5 expression in lymph node T cells from B6 mice (WT), <i>Tcra</i><sup>−/−</s...
<p>A. Electron micrographs of RPEs from positive-strong transgenic mice lines 131, 142, and 157. B. ...
<p>(A) Transgenic Tg(Jpx) and Tg(Jpx, Xist) mouse lines generated in this study. F, female; M, male....
<p>(<b>A</b>) ES cells were transduced with different amounts of lentiviral NT or OT or were not tra...
<p>(A) Schematic map of the microinjected transgene consisting of the α-smooth muscle actin promoter...
<p>A. Schematic representation of the construct used for the generation of the transgenic mice. B. G...
<p>(A) Schematic representation of transgene construction. (B) Southern blot analysis performed on 1...
<p>(A) PCR for detecting TG mice. Two pairs of primers were used to amplify the 489 bp and 282 bp in...
<p><b>A</b>) Representative images of groups of mice (n = 3 per group) transiently transgenized with...