Characterization of T cells from the spleen and lymph node of PbT-I mice.

<p>Cells were harvested from the spleen and the lymph nodes of PbT-I transgenic or littermate control mice (WT). FACS analysis was performed to characterize the expression of CD8, CD4 and the transgenic TCR alpha (Vα8.3) and beta (Vβ10) chains. (A) Representative dot-plots showing the proportions of CD8 versus CD4 cells in the spleen and lymph node of PbT-I and WT mice. (B) Representative histograms showing the expression of the transgenic TCR Vα8.3 and Vβ10 chains on the CD8 or CD4 single-positive cells from the spleen. This experiment was repeated three times with two mice per experiment.