The G1/S cell cycle checkpoint activation of the ULTI mouse urothelial cell line under non-permissive conditions is sensitive to p53 inhibition.

<p>A) Cell cycle analysis by propidium iodide DNA labeling flow cytometry of ULTI cells pretreated with pifithrin-α (PFTa) or DMSO vehicle, then treated with etoposide (ETOP) or DMSO vehicle under non-permissive conditions with 0.5% FBS. B) Quantitation of cell cycle phase of ULTI cells pretreated with pifithrin-α (PFTa) or DMSO vehicle, then treated with etoposide (ETOP) or DMSO vehicle under non-permissive conditions with 0.5% FBS. Dark grey bars indicate percent of cells in G0/G1, white bars indicate percent of cells in S phase, and light grey bars indicate percent of cells in G2/M phase. Error bars represent standard error of the mean.