Expression of WT p53 does not restore p53 activity in MEB-Med8A cells.

<p>(<b>A–C</b>) D283-MED cells were co-transfected with p53-dsRedXP and MDM2-YFP and imaged using time lapse confocal microscopy. The time of etoposide stimulation is represented by the vertical dotted line. The level of p53 and MDM2 were assessed by measuring fluorescence intensity in single cells over time, which were normalised to the baseline fluorescence measured prior to etoposide addition. (B, C): Example of 5 single cell traces and the average fluorescent intensity (red line) of all cells are shown (N = 2, n = 33). (<b>D–F</b>) MEB-Med8A cells were co-transfected and imaged as in (A–C). (E–F): Example of 5 single cell traces and the average fluorescent intensity (red line) of all cells are shown (N = 2, n = 22). (<b>G</b>) A stack column showing the percentage of MB cells with p53 or MDM2 expression above threshold level upon etoposide treatment. Threshold was calculated as average intensity of untreated control +2 SD. D283 cells (N = 2, n = 33); Med8 cells (N = 2, n = 22).