MIF and D-DT promote clonal cell proliferation independent of p53.

<p>A,B: A549 and A549-E6 cells were transfected with siRNA oligos as indicated for 48 h and then re-plated in duplicate in 6 well plates at 500 cells/well. 10–14 days later, colonies were stained with crystal violet, manually enumerated (A) and photographed (B). NS = not significant. (C) H1299 (p53 null) cells were transfected with siRNA oligos as indicated for 48 h and then re-plated in duplicate in 6 well plates at 1000 cells/well. 10–14 days later, colonies were stained with crystal violet, photographed (left panel) and manually quantified (right panel). D: A549 cells were plated in duplicate at 500 cells/well in 6 well plates in the presence of vehicle (0.1% DMSO), 10 µM 4-IPP or 20 µM 4-IPP. Vehicle and 4-IPP were replenished every other day. 10-14 days later, colonies were stained with crystal violet, photographed (left panel) and manually quantified (right panel). Data in all panels are representative of three independent experiments.