The ULTI mouse urothelial cell line has an intact DNA damage response under permissive conditions, but aberrant cell cycle checkpoint and apoptosis activation which normalizes under non-permissive conditions.

<p>A) Western blot of whole cell lysates from NIH 3T3 fibroblasts or ULTI cells under both permissive and non-permissive conditions, pretreated with pifithrin-α or DMSO vehicle, then exposed to etoposide or DMSO vehicle. B) Cell cycle analysis by propidium iodide DNA labeling flow cytometry of ULTI cells treated with etoposide (ETOP) or DMSO vehicle under both permissive conditions with 10% FBS, and non-permissive conditions with 0.5% FBS. C) Quantitation of cell cycle phase of ULTI cells treated with etoposide (ETOP) or DMSO vehicle under both permissive conditions with 10% FBS and non-permissive conditions with 0.5% FBS. Dark grey bars indicate percent of cells in G0/G1, white bars indicate percent of cells in S phase, and light grey bars indicate percent of cells in G2/M phase. Error bars represent standard error of the mean.