Lithium activated Wnt/β -catenin pathway in the SGZ of 3-month-old TgCRND8 mouse brain.

<p>A–D: single β-catenin immunohistochemistry in the saline (A,C)- and lithium (B,D)-treated Tg mouse brain. An apparently higher number of β-catenin positive cells is present in the parietal cortex and dentate gyrus of the hippocampus of lithium-treated Tg mice compared to Tg controls. Scale bar = 50µm (A–B). Scale bar = 100µm (C–D). E–J: triple labelling fluorescence for β-catenin (green), DCX (red) and DAPI (blue) staining. E–G: saline treated Tg mice, G merge of E and F images plus DAPI. H–J lithium-treated Tg mice, J merge of H and I images plus DAPI. White arrows in G and J indicate the nuclear co-localization of DCX and β-catenin staining in the saline-and lithium-treated Tg mice, respectively. Scale bar = 12.5 µm (E–J). sgz: sub granular zone, ctx: cortex; DG: dentate gyrus, Tg: TgCRND8, S = saline, L = lithium.