Effect of lithium treatment on the inactive p-GSK-3β(Ser9) and PHF-1 immunoreactivity in the 7-month-old TgCRND8 mouse brain.

<p>A: qualitative Western blotting and (B,C) densitometric analysis of p-GSK-3β(Ser9) in the cortex and hippocampus of 7-month-old TgCRND8 mice. Statistics indicate a significant increase of p-GSK-3β(Ser9) levels in the neocortex and hippocampus of lithium-treated 7-month-old Tg mice compared with the age-matched controls. *P<0.05, vs saline-treated Tg. Two-way ANOVA plus Bonferroni's post hoc. D: qualitative Western blotting and (E–F) densitometric analysis of PHF-1 protein levels in the cortex and hippocampus of saline- and lithium-treated Tg mice. Statistics indicate a significant reduction of PHF-1 levels in the neocortex and hippocampus of lithium-treated 7-month-old Tg mice compared with the age-matched Tg controls. Data are presented as mean ± S.E.M. *P<0.05, saline-treated Tg vs lithium-treated Tg mice, Student's t-test. β-actin serves as a protein loading control. S = saline, L = lithium, Tg: TgCRND8.