Antigen-specific CD8⁺ T-cell proliferation assay.

<p>Mice were immunized with OVA_257-264-liposome and 1 week or 20 weeks later, CD8⁺ T cells of the immunized mice were cultured in the presence (closed box) or absence (open box) of OVA as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0015091#s4" target="_blank">Materials and Methods</a>. Data represents mean ³H- thymidine incorporation and SE of triplicate cultures. *, significant difference (p>0.01).