Reduced accumulation of antigen-specific CD8⁺ T cells in <i>Hvem</i>^−/− mice.

<p>WT or <i>Hvem</i>^−/− mice were orally infected with 1×10⁹ LM-OVA and the amount of antigen-specific CD8⁺ T cells was measured. A- Percentage and absolute number of OVA-specific CD8⁺ T cells in splenocytes isolated from WT or <i>Hvem</i>^−/− mice, 7 days p.i. B- IFNγ production by WT or <i>Hvem</i>^−/− splenic CD8⁺ T cells after <i>ex vivo</i> restimulation with OVA_257–264 peptide. The percentage of OVA-specific CD8⁺ T cells in the MLN (C) and IEL (D) of WT and <i>Hvem</i>^−/− mice was also calculated, 7 days after infection. Data shown correspond to 6–9 mice per group, pooled from two independent experiments. E- Percentage and absolute number of memory OVA-specific CD8⁺ T cells in the spleen of WT and <i>Hvem</i>^−/− mice, 70 days p.i. F- IFNγ production by OVA-specific memory CD8⁺ T cells isolated from the spleen of WT and <i>Hvem</i>^−/− mice 70 days p.i. and restimulated <i>ex vivo</i> with OVA-peptide. Data were pooled from at least two independent experiments, each one performed with 5–7 mice per group.