Dose-response of cytokine production by CD8⁺ T cell and CTL induction in mice immunized with OVA_257-264-liposome or with OVA_257-264 solution.

<p>A serial two-fold dilution of OVA_257-264-liposome (open box) and OVA_257-264 solution (closed box) were made in PBS, and mice were immunized with the diluents in the presence of 5 µg CpG. OVA_257-264 solution containing equal amounts of peptides as those in OVA_257-264-liposome. One week after the immunization, IFN-γ production by CD8⁺ T cells (<b>A</b>) and the CTL response (<b>B</b>) were monitored as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0015091#s4" target="_blank">Materials and Methods</a>. Data represent means and SE of three mice per group. *, significant difference (p>0.01).