Golgi staining and Sholl analysis of pyramidal neurons in CA1.

<p>(A) A camera lucida drawing of a pyramidal neuron and superimposed concentric circles used for Sholl analysis. The radius interval between circles was 20 µm per step, ranging from 20 µm to 300 µm from the center of the neuronal soma. Ranges analyzed for dendritic length: 20–100 µm range (dark grey zone), 120–200 µm range (light grey zone) and 220–300 µm (white zone). (B) Quantification of the number of intersections per circle. Significant differences were found in the circles ranging from 20 µm to 100 µm in DIAB-SC compared with CTL-SC (*** <i>p</i><0.001) and an increase when comparing DIAB-EE with DIAB-SC at 20–140 µm (* <i>p</i><0.05). (C) Quantification of the dendritic length of CA1 pyramidal neurons for the three distance ranges studied: 20–100 µm, 120–200 µm and 220–300 µm. Significant differences were found in the 20–100 µm range with a decrease in DIAB-SC mice (* <i>p</i><0.05 vs. CTL-SC) and an increase in the DIAB-EE group (** <i>p</i><0.01 vs. DIAB-SC). A main effect of ‘housing’ was found at 120–200 µm and 220–300 µm ranges (<i>p</i><0.01 and <i>p</i><0.05 respectively). (D) Camera lucida drawings of pyramidal neurons representative of each experimental group. Scale bar corresponds to 50 µm.