Dendritic branching and distribution of postsynaptic spines and presynaptic boutons in CA3 pyramidal neurons of control and mutant mice.

<p>A, Examples of three-dimensional reconstruction using IMARIS and FilamentTracer software. B, Three-dimensional reconstruction of AAV-EGFP-infected CA3 pyramidal neurons. Graphs represent the numbers of primary, secondary and tertiary dendrites of CA3 pyramidal neurons in control (open boxes, <i>n</i> = 10–12) and mutant mice (filled boxes, <i>n</i> = 6–7). There were no significant differences between control and mutant mice in the numbers of primary (apical, <i>P</i> = 0.58; basal, <i>P</i> = 0.06; <i>t</i>-test), secondary (<i>P</i> = 0.13, <i>P</i> = 0.21) and tertiary dendrites (<i>P</i> = 1.0 <i>P</i> = 0.16). C, Tertiary dendritic segments in control (left, top) and mutant (left, bottom) mice. Normalized distribution of inter-spine distances (middle, bin size, 0.1 µm). Cumulative distribution of inter-spine distances (right, same data set). There were no significant differences in inter-spine intervals of CA3 pyramidal neurons between two genotypes (control <i>n</i> = 428 from 10 dendrites of 4 mice; mutant, <i>n</i> = 459 from 9 dendrites of 4 mice; <i>P</i> = 0.74, Kolmogorov-Smirnov test). D, Boutons on the axon in the CA3 stratum radiatum of control (left, top) and mutant (left, bottom) mice. Normalized distribution of inter-bouton distances (middle, bin size, 0.4 µm). Cumulative distribution of inter-bouton distances (right, same data set). There were no significant differences in inter-bouton intervals of CA3 pyramidal neurons between two genotypes (control <i>n</i> = 262 from 18 axons of 4 mice; mutant, <i>n</i> = 322 from 24 dendrites of 4 mice; <i>P</i> = 0.90, Kolmogorov-Smirnov test).