SDS-PAGE of terminal deletion variants of <i>Hs</i>mtSSB.

<p>Near-homogeneous fractions (∼2 µg) of recombinant <i>Hs</i>mtSSBwt (<i>lane 1</i>), ΔN (<i>lane 2</i>), ΔC (<i>lane 3</i>), and ΔNΔC (<i>lane 4</i>) were subjected to SDS-PAGE in a 17% gel, followed by Coomassie blue staining as described under “<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0015379#s4" target="_blank">Materials and Methods</a>.” The sizes of molecular mass markers (BenchMark™ Pre-Stained Protein Ladder, Invitrogen™) are indicated in kDa at <i>left</i>.