<p>SDS-PAGE of decreasing amounts of whole FBS (lanes 1–5), and HS (lanes 6–10). FBS or HS was used at 1∶100 dilution, with the following amounts of protein loaded onto each lane: lanes 1–5: 4 µg, 3.5 µg, 3 µg, 2.5 µg, and 1.5 µg, respectively; and lanes 6–10: 3 µg, 1.8 µg, 1.2 µg, 0.6 µg, and 0.3 µg, respectively. The white dots shown in lanes 5 and 10 correspond to the bands excised for protein identification by MALDI-TOF mass fingerprint analysis.</p
<p>The middle lane was a PageRuler Prestained Protein Ladder, with the sizes indicated in kDa. The n...
<p>Lane 1, molecular mass markers with the sizes shown on the left in kDa; lanes 2–9, SmDHAR1, SmDHA...
<p>A) Protein purity assessed on SDS-PAGE. MW: molecular weight standard from Euromedex; 1: Cfp21 WT...
<p>(A) Serum granulations (pellets) were prepared exactly as in <a href="http://www.plosone.org/arti...
<p>Note: The MALDI-TOF-MS peptide mass fingerprint spectrum of trypsin-digested protein (a) and its ...
<p>Lanes 1, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 and 24 are crude plasma samples from subjects enroll...
<p>Proteins (0.7 mg/ml) were incubated for 3 h without Abs (odd numbers), 0.03 mg/ml csf-IgG<sub>mix...
<p>Proteins (∼2 µg per lane) were reduced with 5% BME or loaded on a 12% gel in non-reducing conditi...
<p>Near-homogeneous fractions (∼2 µg) of recombinant <i>Hs</i>mtSSBwt (<i>lane 1</i>), ΔN (<i>lane 2...
<p><i>Lanes 1–8,</i> eight bands of proteins with the apparent molecular masses of 150 (SSCL-150), 1...
<p>(A) Stack view of the 4210 Da protein in four groups analyzed by ClinProTools™ 2.1 software (red ...
<p>Lane 1: protein standard; Lane 2: R18; Lane 3: R43. Powdered enzyme (100 µg) was dissolved in dis...
<p>Purified refolded-α2 was loaded in SDS-PAGE gels, the two bands (α2 and α2′) observed after stain...
a<p>The values shown for each protein correspond to the number of unique ...
<p>The lanes were loaded as follows: lane M, protein molecular weight standards; lane 1, purified Li...
<p>The middle lane was a PageRuler Prestained Protein Ladder, with the sizes indicated in kDa. The n...
<p>Lane 1, molecular mass markers with the sizes shown on the left in kDa; lanes 2–9, SmDHAR1, SmDHA...
<p>A) Protein purity assessed on SDS-PAGE. MW: molecular weight standard from Euromedex; 1: Cfp21 WT...
<p>(A) Serum granulations (pellets) were prepared exactly as in <a href="http://www.plosone.org/arti...
<p>Note: The MALDI-TOF-MS peptide mass fingerprint spectrum of trypsin-digested protein (a) and its ...
<p>Lanes 1, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 and 24 are crude plasma samples from subjects enroll...
<p>Proteins (0.7 mg/ml) were incubated for 3 h without Abs (odd numbers), 0.03 mg/ml csf-IgG<sub>mix...
<p>Proteins (∼2 µg per lane) were reduced with 5% BME or loaded on a 12% gel in non-reducing conditi...
<p>Near-homogeneous fractions (∼2 µg) of recombinant <i>Hs</i>mtSSBwt (<i>lane 1</i>), ΔN (<i>lane 2...
<p><i>Lanes 1–8,</i> eight bands of proteins with the apparent molecular masses of 150 (SSCL-150), 1...
<p>(A) Stack view of the 4210 Da protein in four groups analyzed by ClinProTools™ 2.1 software (red ...
<p>Lane 1: protein standard; Lane 2: R18; Lane 3: R43. Powdered enzyme (100 µg) was dissolved in dis...
<p>Purified refolded-α2 was loaded in SDS-PAGE gels, the two bands (α2 and α2′) observed after stain...
a<p>The values shown for each protein correspond to the number of unique ...
<p>The lanes were loaded as follows: lane M, protein molecular weight standards; lane 1, purified Li...
<p>The middle lane was a PageRuler Prestained Protein Ladder, with the sizes indicated in kDa. The n...
<p>Lane 1, molecular mass markers with the sizes shown on the left in kDa; lanes 2–9, SmDHAR1, SmDHA...
<p>A) Protein purity assessed on SDS-PAGE. MW: molecular weight standard from Euromedex; 1: Cfp21 WT...