Impairment of FasL-induced caspase activation and apoptosis in caspase-8 and -10-doubly deficient Jurkat cells.

<p><b>A, B</b>, A3 (white bars), I9-2d (grey bars) and I9-2e (black bars) Jurkat cells were incubated for 8 hours in the presence or absence of 500 ng/mL FasL as indicated. Caspase activities were assessed using Ac-DEVD-AMC or Ac-IETD-AMC (<b>A</b>). Cells were stained with annexin-V-FITC and propidium iodide and analyzed by flow cytometry. Percentages of annexin-V-positive (AnV+) are indicated (<b>B</b>). All data are means ± SEM of three to four independent experiments. *p<0.05; **p<0.01, *** p<0.001. <b>C</b>, Representative flow cytometry experiment. Low right quadrants: percentages of [AnV-FITC+ propidium iodide (PI)-] cells; Up right quadrants: percentages of [AnV-FITC+ PI+] cells; Up left quadrants: percentages of [AnV-FITC- PI+] cells.