Caspase-8 and -10 can restore FasL-induced apoptosis in caspase-8 and -10-doubly deficient Jurkat cells.

<p><b>A, B</b>, I9-2e Jurkat cells were transiently transfected with plasmids encoding EGFP, EGFP-tagged wild-type caspase-8 or -10 or EGFP-tagged catalytically inactive caspase-8 mutant (Casp-8 C360S). Alternatively, cells were co-transfected with plasmids encoding EGFP and catalytically inactive caspase-10 mutant (C401S). Twelve hours post-transfection, cells were further incubated for 4 hours in the presence or absence of 500 ng/mL FasL. Cells were labelled with annexin-V-APC (AnV-APC). EGFP fluorescence was monitored on untreated cells to determine the percentage of transfected cells (A). Analysis of annexin-V binding was restricted to the EGFP-expressing cells on both untreated (None) and FasL-treated (FasL) cells (B). Percentages of fluorescent cells are indicated. Data are from a representative experiment out of three independent experiments. <b>C</b>, Specific cell death triggered by FasL was calculated by subtracting basal values obtained in the absence of FasL for each transfection. Data are means ± SEM of three independent experiments. *p<0.05; **p<0.01.