Impairment of FasL-induced cell death in caspase-8 and -10-doubly deficient Jurkat cells.

<p><b>A</b>, 20 µg total protein extracts from parental (A3) and caspase-8 deficient (I9-2a, b, d and e) Jurkat cell lines were subjected to SDS-PAGE, and Western blotted with anti-caspase-8, -10, -2, -9, -3, -7, anti-FADD, anti-RIP or anti-β-actin antibodies. B, Jurkat cells were labelled with anti-CD95-PE antibody (thick line) or an isotype control (thin line) and analysed by flow cytometry. Percentages of cells expressing CD95 are indicated. C, Jurkat cells were incubated for 8 hours in the presence of 500 ng/mL FasL and cell viability was evaluated by MTT assay. Values are means ± SEM of three independent experiments. *p<0.05; **p<0.01, *** p<0.001 as compared to values obtained in A3 cells.