<p>A large population of pollen tubes of RNAi tobacco(A) compared with wild-type pollen tubes(B) to illustrate the difference of both length and number of pollen tubes; C: Western-blotting with anti-NtGNL1 to confirm the RNAi effects on pollen. NtGNL1 expression in RNAi lines. Anti-actin was used as an endogenous standard; wild-type SR1 was used as control. Bar = 100 µm.</p
<p>Total RNA was isolated from inoculated BY-2 protoplasts at 18 hpt. Mutants used for inoculation a...
Abstract Genetically engineered pollen with a visible marker gene could be useful to monitor the mov...
RNA silencing is a mechanism of gene regulation by sequence specific RNA degradation and is involved...
Antisense oligodeoxynucleotide (A-ODN) inhibition works well in animal cells. However, there have be...
Antisense oligodeoxynucleotide (A-ODN) inhibition works well in animal cells. However, there have be...
<p><b>A–E:</b> controls (wild-type pollen tubes) showing a strict time sequence of FM4-64 uptake wit...
In seed plants, successful fertilization requires correct regulation of pollen tube growth. At germi...
<p>A: Inhibition effects among antisense ODNs (1.0 µM). n = 300±10. Pollen tubes under ON4 and ON6 t...
<p>RNAi constructs were generated for all candidate <i>CysP</i>s and the presence of <i>CysP</i> dou...
The effects of an animal RNase fused to the late cotton pollen-specific promoter G9 in a plant syste...
<p>(A) Regions of proteinases genes used in RNAi experiments. Numbers indicate nucleotide positions....
The RNA interference is a mechanism, which allows cells to regulate their genes functions, to establ...
Background Pollen tube growth is essential for plant reproduction and represents a widely employe...
<p>GFP transgenes with pollen-specific expression were used to assay the activity of endogenous siRN...
RNA interference (RNAi) is an important mechanism regulating gene expression. In plants, RNAi is tri...
<p>Total RNA was isolated from inoculated BY-2 protoplasts at 18 hpt. Mutants used for inoculation a...
Abstract Genetically engineered pollen with a visible marker gene could be useful to monitor the mov...
RNA silencing is a mechanism of gene regulation by sequence specific RNA degradation and is involved...
Antisense oligodeoxynucleotide (A-ODN) inhibition works well in animal cells. However, there have be...
Antisense oligodeoxynucleotide (A-ODN) inhibition works well in animal cells. However, there have be...
<p><b>A–E:</b> controls (wild-type pollen tubes) showing a strict time sequence of FM4-64 uptake wit...
In seed plants, successful fertilization requires correct regulation of pollen tube growth. At germi...
<p>A: Inhibition effects among antisense ODNs (1.0 µM). n = 300±10. Pollen tubes under ON4 and ON6 t...
<p>RNAi constructs were generated for all candidate <i>CysP</i>s and the presence of <i>CysP</i> dou...
The effects of an animal RNase fused to the late cotton pollen-specific promoter G9 in a plant syste...
<p>(A) Regions of proteinases genes used in RNAi experiments. Numbers indicate nucleotide positions....
The RNA interference is a mechanism, which allows cells to regulate their genes functions, to establ...
Background Pollen tube growth is essential for plant reproduction and represents a widely employe...
<p>GFP transgenes with pollen-specific expression were used to assay the activity of endogenous siRN...
RNA interference (RNAi) is an important mechanism regulating gene expression. In plants, RNAi is tri...
<p>Total RNA was isolated from inoculated BY-2 protoplasts at 18 hpt. Mutants used for inoculation a...
Abstract Genetically engineered pollen with a visible marker gene could be useful to monitor the mov...
RNA silencing is a mechanism of gene regulation by sequence specific RNA degradation and is involved...