The effect of A-ODNs on pollen tube growth and <i>NtGNL1</i> expression level.

<p>A: Inhibition effects among antisense ODNs (1.0 µM). n = 300±10. Pollen tubes under ON4 and ON6 treatment were obvious shorter than that under other treatment. Asterisks indicate a significant difference (<i>P</i><0.05). These data were calculated and analyzed by SPSS (16.0) Independent-Sample T Test. Error bars in the columns represent SD. B: No significant inhibition effect on pollen tubes growth was observed during treatment by sense and random ODNs. n = 300±10. C: the effect of antisense ODN treatment on <i>NtGNL1</i> mRNA expression. D: the comparison of cytotoxic effect between control (sense or nonsense) and antisense ON4. All of them displayed around 80% viability. n = 300±10.