Silencing of B5 blocks viral protein expression.

<p>(A). CaSki cells were transfected with siB5 or siHVEM and then 48 h later, synchronously infected with HSV-2(G) and nuclear extracts harvested at the indicated times and evaluated for VP16. Blots are representative of results obtained in 3 independent experiments. (B). Transfected cells were infected with HSV-1(KOS) and 4 h pi, cellular lysates harvested and analyzed for the expression of ICP4, ICP0 and ICP27. Blots were scanned and the relative expression of viral proteins by odu is presented as a percentage of expression in cells transfected with siHVEM obtained in 3 independent experiments (right).