HSV-1 infection induces Hsp90 upregulation and nuclear translocation.

<p>(A) MRC-5 cells were infected with HSV-1 (MOI = 10) for 0, 1, 2, 4, or 6 h. The cells were then harvested, lysed, and analyzed for total Hsp90 expression. The Western blotting results shown in the line graph were normalized to GAPDH expression and were expressed as the fold increase relative to the cell control. Each value represents the mean ± SD of three independent experiments. (B) MRC-5 cells infected with HSV-1 (MOI = 10) for 4 h were fixed, permeabilized and stained for ICP5 (red), total Hsp90 (green), and nuclei (blue). (C) Interaction between ICP5 and Hsp90. Cells transfected with or without pEGFP-Hsp90 were lysed, immunoprecipitated with anti-Hsp90 antibody and probed with indicated antibodies. Non-capsid protein ICP27 was used as a negative control.