Decreased HSV-1 viral protein expression can be observed in unphosphorylatable BAF mutant cells.

<p>Western blot analysis of HSV-1 viral proteins upon infection of FLAG-BAF-MTTSQ and FLAG-BAF-MAAAQ cells. Cells were infected with HSV-1 virus at MOI = 1 for 20 h. Following cell harvest, protein samples were resolved by SDS-PAGE and transfer to membrane. Membranes were incubated with antibodies against HSV-1 proteins: ICP0, ICP4, ICP27, ICP8, TK, gD, and VP16. Anti-tubulin antibody was used as a loading control. Data were obtained from three independent experiments and a representative blot is shown. Values represent average relative signal intensity from three experiments as quantified by ImageLab software (BioRad).