Oleic acid is unable to fully reverse the low proliferation rate of SCD1-deficient when de novo lipid synthesis is blocked.

<p>H460 lung cancer cells were treated with 1 µM CVT-11127 (CVT), 10 µM CP-640186 or both in presence or absence of 100 µM palmitic acid (Pal) or oleic acid (Ole) (<b>A</b>), and cell proliferation was assessed after 48 h. Distribution of cells in cell cycle phases was determined by fluorocytometry in cells incubated with CP-640186 plus or minus 100 µM oleic acid and (<b>B</b>). Cancer cell proliferation was also determined upon treatment with 1 µM CVT-11127 (CVT), 20 µM CP-640186, or both in presence or absence of 100 µM palmitic acid (Pal) or oleic acid (Ole) (<b>C</b>), and in cells treated for 48 h with 1 µM CVT-11127, 25-hydroxycholesterol or both, plus or minus 100 µM oleic acid (<b>D</b>). In all experiments, control cells received equivalent volumes of DMSO vehicle. <b>*</b>, p<0.05 or less compared to DMSO; **, p<0.05 or less compared to CVT-treated control, by Student's t test.