Viral DNA analysis in <i>N. benthamiana</i> and Arabidopsis plants agro-inoculated with wild-type and mutated BSCTV.

<p>(A) Southern blot analysis of total nucleic acids extracted from agro-inoculated (0 and 2d) and newly emerged (0, 2, 8, 10, 13, 16 and 20d) leaves of <i>N. benthamiana</i> after agro-inoculation. (B) Southern blot analysis of total nucleic acids extracted from newly emerged leaves of Arabidopsis two weeks after agro-inoculation. Size marker (the first lane on the left) was a mixture of BSCTV fragments digested by <i>Tth</i>111I and <i>Eco</i>RI, and the fragment digested by <i>Eco</i>RI. Fragment sizes are given in kb. The positions of open circle (oc), linear (lin), supercoiled (sc) and single stranded (ss) DNAs, and a population of subgenomic DNA forms are indicated.