Testing of activity of AV3 promoter in <i>N. benthamiana</i> by 5′ RACE and <i>Agrobacterium</i>-mediated inoculations.

<p><i>N. benthamiana</i> agro-infiltrated with infectious clones of wild type AYVV-NT, pAY1-7 (A), or mutant pAYAV3PM-DI (B), were used for mapping of the 5′-ends of virion-sense mRNA transcripts by 5′ RACE. The numbers of clones with specific 5′-terminus are shown in the bar chart. X-axis, the nucleotide positions in AYVV-NT genome; Y-axis, clone counts. (C) Symptoms induced by wild type and mutant AYVV-NT. Whole plant image of the healthy and inoculated <i>N. benthamiana</i> plants infiltrated with <i>A. tumefaciens</i> harboring AYVV-NT wild type (pAY1-7) and mutants (pAYAV3PM-DI) at 21 dpi is shown.