<p>(A and B) Non-reducing and reducing immunoblot of periplasmic extract from overnight expression of 4G2 in HB2151 (HB) and BL21(DE3) (BL) <i>E. coli</i> strains, respectively. All blots were probed with anti-Human Fc-HRPO.</p
After electrophoresis of an alkaline extract of type 15 group A streptococci, three main precipitati...
<p>a) The culture-adapted Kenyan isolate 9197 was selected three times with anti-human IgM coated Dy...
<p>Bacteria prepared as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.002...
<p>(A-E) Left and middle panels: Non-reducing immunoblot of 7.5 µl clarified cell lysate from small ...
<p>Soluble hemagglutinins were prepared from the supernatant of transfected HEK 293T cells, after se...
<p>(<b>A</b>) Western blots showing co-immunoprecipitation of biotinylated proteins by anti-CagI ant...
M: Protein ladder, Lane 1: anti- PD-1, Lane 2: CD80-Fc, and Lane 3: 4-1BBL-Fc.</p
<p>The strain used for co-IP expressed the respective proteins in suitable amounts (cell extracts); ...
<p>(A) Representative western blots showing the expression profile of the TCR formats in either <i>E...
<p>(A). Non-reducing SDS-PAGE of 2 µg of pooled bacterial IgG eluate after protein L purification (E...
Bacterial expression systems can greatly facilitate protein engineering of antibodies. We have devel...
<p>Flow cytometric analysis was used to quantify antibody binding to bacterial cells. Representative...
<p>Time course of expression of Hfq-Flag during growth in LB at 37°C (A) and at 27°C (B). (A) Bacter...
<p>Native PAGE Western blot analysis with anti-His<sub>6</sub>-tag antibody of detergent-free bacter...
After electrophoresis of an alkaline extract of type 15 group A streptococci, three main precipitati...
After electrophoresis of an alkaline extract of type 15 group A streptococci, three main precipitati...
<p>a) The culture-adapted Kenyan isolate 9197 was selected three times with anti-human IgM coated Dy...
<p>Bacteria prepared as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.002...
<p>(A-E) Left and middle panels: Non-reducing immunoblot of 7.5 µl clarified cell lysate from small ...
<p>Soluble hemagglutinins were prepared from the supernatant of transfected HEK 293T cells, after se...
<p>(<b>A</b>) Western blots showing co-immunoprecipitation of biotinylated proteins by anti-CagI ant...
M: Protein ladder, Lane 1: anti- PD-1, Lane 2: CD80-Fc, and Lane 3: 4-1BBL-Fc.</p
<p>The strain used for co-IP expressed the respective proteins in suitable amounts (cell extracts); ...
<p>(A) Representative western blots showing the expression profile of the TCR formats in either <i>E...
<p>(A). Non-reducing SDS-PAGE of 2 µg of pooled bacterial IgG eluate after protein L purification (E...
Bacterial expression systems can greatly facilitate protein engineering of antibodies. We have devel...
<p>Flow cytometric analysis was used to quantify antibody binding to bacterial cells. Representative...
<p>Time course of expression of Hfq-Flag during growth in LB at 37°C (A) and at 27°C (B). (A) Bacter...
<p>Native PAGE Western blot analysis with anti-His<sub>6</sub>-tag antibody of detergent-free bacter...
After electrophoresis of an alkaline extract of type 15 group A streptococci, three main precipitati...
After electrophoresis of an alkaline extract of type 15 group A streptococci, three main precipitati...
<p>a) The culture-adapted Kenyan isolate 9197 was selected three times with anti-human IgM coated Dy...
<p>Bacteria prepared as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.002...