Native Western blot analysis of bacterial membrane fractions.
- Roberto Jappelli (510420)
- Marilyn H. Perrin (510421)
- Kathy A. Lewis (510422)
- Joan M. Vaughan (510423)
- Christos Tzitzilonis (275867)
- Jean E. Rivier (317493)
- Wylie W. Vale (293991)
- Roland Riek (39957)
Publication date
January 2014
DOIAbstract
<p>Native PAGE Western blot analysis with anti-His<sub>6</sub>-tag antibody of detergent-free bacterial membrane fractions, which derived from the expression of PelB-hCRFR1α (R1) and PelB-mCRFR2β (R2) carried out in Rosetta2(DE3) strain and TB medium. Vector pET-26b (-) was used as negative control. The 1,048 kDa band corresponds to the smallest protein (IgM pentamer) present in the NativeMark Unstained Protein Standard (Life Technologies), which is detected by the antibody.</p
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