Immunodetection of Hfq-Flag in total protein extracts of <i>Y</i>. <i>enterocolitica</i>.

<p>Time course of expression of Hfq-Flag during growth in LB at 37°C (A) and at 27°C (B). (A) Bacterial extracts of WA^RS derivatives (odd-numbered lanes) or JB580v derivatives (even-numbered lanes) were prepared after 2, 4, 6, 8 and 24 h of growth at 37°C. Loading was as follows: extracts from SOR33 in lanes 1, 3, 5, 7 and 9; SOR35 in lanes 2, 4, 6, 8 and 10; parental strain WA^RS in lanes 11 and 13; and parental strain JB580v in lanes 12 and 14. The upper band indicated by an asterisk is a background band also present in parental strains WA^RS and JB580v (lanes 11–14) and it was used as loading control. (B) Bacterial extracts of JB580v derivatives were prepared after growth for 2, 3, 4, 6, 8 and 12 h at 27°C. Loading was as follows: extracts from SOR35 in lanes 1–6 and parental strain JB580v in lane 7.