Immunohistochemistry analysis of RCS cells expressing MT-COMP with and without a COMP-directed shRNA 3B.

<p>RCS cells with (right panel) and without (left panel) shRNA 3B, grown on coverslips, were infected with adenovirus expressing MT-COMP-FLAG and GFP. Cells were fixed 4 days after adenoviral infection and immunostained with COMP, type IX collagen, MATN3 and type II collagen antibodies as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0010302#s4" target="_blank">Methods</a> section. GFP was used to identify infected cells. FLAG was used as a marker for recombinant COMP (red) and the rER was delineated with a CRT antibody (blue).