Cells expressing MT-COMP with a COMP-directed shRNA 3B show reduction of intracellular COMP and CRT.

<p>COS-7 cells with (<b>I–L</b>) and without (<b>E–H</b>) shRNA 3B were infected with adenoviruses expressing MT-COMP (<b>E–L</b>). Uninfected COS-7 cells with shRNA 3B serves as controls (<b>A–D</b>). Cells were grown on coverslips for 48 hours after adenoviral infection and immunostained with COMP and CRT antibodies as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0010302#s4" target="_blank">Methods</a> section. DAPI (blue) staining was used to localize all of the cells in the field. COMP (green in E) co-localizes (merge-yellow in H) with CRT (red in F).