Construction and analysis of a pIX-MT containing adenovirus.
- J. Michael Mathis (53440)
- Shilpa Bhatia (303901)
- Alok Khandelwal (356287)
- Imre Kovesdi (303922)
- Stephen J. Lokitz (319044)
- Yoshi Odaka (356288)
- Amol M. Takalkar (356289)
- Tracee Terry (319047)
- David T. Curiel (120894)
Publication date
February 2013
DOIAbstract
<p>(A) Schematic representation of pIX-MT and a CMV-tGFP expression cassette inserted into the Ad5 genome. (B) Expression of GFP in cells infected with Ad-tGFP-pIX-MT shows formation of an individual plaque. (C) Detection of adenovirus fiber and metallothionein proteins incorporated into the purified Ad-tGFP-pIX-MT and Ad-CMV-EGFP viral particles by western blot analysis.</p
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